One of the current challenges faced by the organoid technology is their high variability and lack of tools for single cell real-time analysis. My team focuses on combining innovations in biosensors, fluorescence lifetime imaging microscopy (FLIM) and biofabrication fields to controllably produce intestinal organoid cultures with known and controllable growth and metabolic characteristics. To solve this challenge, we develop multi-parametric 3D live imaging methodologies addressing analysis of organoid oxygenation, visualization of stem cell niche, labelling of specific cell types, their proliferation and metabolism. Using this approach we aim to design ‘active’ biosensors scaffold materials, understand gut-microbiota interactions and physiological role of O2 gradients in the intestine, in health and disease.